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Product Info Summary
| SKU: | M00137 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD14 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00137
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD14 Rabbit Monoclonal Antibody catalog # M00137. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD14 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00137)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FID-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD14
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00137 is reactive to CD14 in Human
Applications
M00137 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
25 kDa
Calculated molecular weight
40.076kDa
Background of CD14
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human breast, using CD14 Antibody.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of K562 cells, using CD14 Antibody.
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Western blot analysis of CD14 expression in Human tonsil cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CD14 (Source: Uniprot.org, NCBI)
Gene Name
CD14
Full Name
Monocyte differentiation antigen CD14
Weight
40.076kDa
Alternative Names
CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein CD14 CD14 molecule monocyte differentiation antigen CD14|myeloid cell-specific leucine-rich glycoprotein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD14, check out the CD14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD14 Rabbit Monoclonal Antibody (M00137)
Hello CJ!
No publications found for M00137
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-CD14 Rabbit Monoclonal Antibody
Question
Is this M00137 anti-CD14 Rabbit Monoclonal antibody reactive to the isotypes of CD14?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
The immunogen of M00137 anti-CD14 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD14. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-31
Question
Our lab were well pleased with the WB result of your anti-CD14 Rabbit Monoclonal antibody. However we have seen positive staining in glioblastoma cell membrane using this antibody. Is that expected? Could you tell me where is CD14 supposed to be expressed?
S. Wu
Verified customer
Asked: 2019-12-23
Answer
According to literature, glioblastoma does express CD14. Generally CD14 expresses in cell membrane. Regarding which tissues have CD14 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebrospinal fluid, Pubmed ID: 19838169
Glioblastoma, Pubmed ID: 2779588
Liver, Pubmed ID: 19159218, 24275569
Lymphocyte, Pubmed ID: 2453848
Macrophage, Pubmed ID: 2472171
Plasma, Pubmed ID: 16335952
Promyelocytic leukemia, Pubmed ID: 18810425
Boster Scientific Support
Answered: 2019-12-23
Question
Our lab used your anti-CD14 Rabbit Monoclonal antibody for IF on lymphocyte in a previous project. I am using human, and I plan to use the antibody for WB next. I would like examining lymphocyte as well as macrophage in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
I have checked the website and datasheets of our anti-CD14 Rabbit Monoclonal antibody and I see that M00137 has been tested on human in both IF and WB. Thus M00137 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-08
Question
We are currently using anti-CD14 Rabbit Monoclonal antibody M00137 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
The anti-CD14 Rabbit Monoclonal antibody (M00137) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-06
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for promyelocytic leukemia using anti-CD14 Rabbit Monoclonal antibody M00137. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-04
Question
We have observed staining in human liver. Are there any suggestions? Is anti-CD14 Rabbit Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
Based on literature liver does express CD14. Based on Uniprot.org, CD14 is expressed in liver, lymphocyte, macrophage, promyelocytic leukemia, brain, glioblastoma, plasma, cerebrospinal fluid, among other tissues. Regarding which tissues have CD14 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebrospinal fluid, Pubmed ID: 19838169
Glioblastoma, Pubmed ID: 2779588
Liver, Pubmed ID: 19159218, 24275569
Lymphocyte, Pubmed ID: 2453848
Macrophage, Pubmed ID: 2472171
Plasma, Pubmed ID: 16335952
Promyelocytic leukemia, Pubmed ID: 18810425
Boster Scientific Support
Answered: 2019-06-11
Question
Do you have a BSA free version of anti-CD14 Rabbit Monoclonal antibody M00137 available?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD14 Rabbit Monoclonal antibody M00137 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-28
Question
Will M00137 anti-CD14 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
As indicated on the product datasheet, M00137 anti-CD14 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-14
Question
Will anti-CD14 Rabbit Monoclonal antibody M00137 work for IF with promyelocytic leukemia?
Verified Customer
Verified customer
Asked: 2018-10-12
Answer
According to the expression profile of promyelocytic leukemia, CD14 is highly expressed in promyelocytic leukemia. So, it is likely that anti-CD14 Rabbit Monoclonal antibody M00137 will work for IF with promyelocytic leukemia.
Boster Scientific Support
Answered: 2018-10-12
Question
I was wanting to use your anti-CD14 Rabbit Monoclonal antibody for IF for human promyelocytic leukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human promyelocytic leukemia identification?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
As indicated on the product datasheet, M00137 anti-CD14 Rabbit Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human promyelocytic leukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-09
Question
Here is the WB image, lot number and protocol we used for promyelocytic leukemia using anti-CD14 Rabbit Monoclonal antibody M00137. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-31
Question
Is a blocking peptide available for product anti-CD14 Rabbit Monoclonal antibody (M00137)?
B. Moore
Verified customer
Asked: 2017-03-21
Answer
We do provide the blocking peptide for product anti-CD14 Rabbit Monoclonal antibody (M00137). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-03-21
Question
you antibody to test anti-CD14 Rabbit Monoclonal antibody M00137 on human promyelocytic leukemia for research purposes, then I may be interested in using anti-CD14 Rabbit Monoclonal antibody M00137 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Miller
Verified customer
Asked: 2016-03-24
Answer
The products we sell, including anti-CD14 Rabbit Monoclonal antibody M00137, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-03-24
Question
I see that the anti-CD14 Rabbit Monoclonal antibody M00137 works with IF, what is the protocol used to produce the result images on the product page?
S. Parker
Verified customer
Asked: 2015-06-04
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-06-04
Question
My question regarding product M00137, anti-CD14 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Lewis
Verified customer
Asked: 2014-02-20
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00137 anti-CD14 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-02-20
Question
Does anti-CD14 Rabbit Monoclonal antibody M00137 work on bovine WB with brain?
E. Bhatt
Verified customer
Asked: 2013-08-21
Answer
Our lab technicians have not tested anti-CD14 Rabbit Monoclonal antibody M00137 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CD14 Rabbit Monoclonal antibody M00137 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-08-21
